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In vitro biotinylation assay DNA fragments encoding HA-TurboID with T7-promoter and SD-sequence were amplified from pCDNA3-mem-Turbo (Gift from Jonathan Long, Addgene plasmid number 149409), with primers 5-GTTTAACTTTAAGAAGGAGATATACCAATGTA CCCATACGATGTTCCAGATTACGCT-3, 5-TAATACGACTCACTATAGGGTTTT GTTTAACTTTAAGAAGGAGATATACCAATG-3 and 5-GAGCTAACGTGGCTT CTTCTGCCAA-3)

GSH depletion induced autophagy accompanied by ferroptosis, as well as cell senescence

The synthesis of metal QCDs, particularly those incorporating noble metals like gold and silver, is expensive due to the cost of raw materials [26]